Anti-ERK1 (phospho Tyr204) Antibody (A16536)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-ERK1 (phospho Tyr204) Antibody (A16536) at 1:1,000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-ERK1 (phospho Tyr204) Antibody (A16536) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human uterus using Anti-ERK1 (phospho Tyr204) Antibody (A16536) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-ERK1 (phospho Tyr204) Antibody (A16536) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.