Western blot analysis of various lysates, using Anti-ESE1 Antibody (A89960) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit. Exposure time: 60s.
Immunoprecipitation analysis of 150µg extracts of A549 cells using 3µg of Anti-ESE1 Antibody (A89960). This Western blot was performed on the immunoprecipitate using Anti-ESE1 Antibody (A89960) at a dilution of 1:500. Detection was with a ECL Enhanced Kit. Exposure time: 30s.
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