Anti-Estrogen Receptor alpha Antibody [6F11] (A279486)

Anti-Estrogen Receptor alpha Antibody [6F11]

Mouse monoclonal [6F11] antibody to Estrogen Receptor alpha.

This antibody recognises the human estrogen receptor alpha chain (ERa), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERa is a ~65 kDa steroid hormone receptor containing an N-terminal (AF-1) ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an (AF-2) domain. ERa binds to DNA as a homodimer (Klinge 2001) and can also form heterodimers with Estrogen receptor beta. The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy. Mouse anti human estrogen alpha antibody, clone 6F11 has been used successfully for identification of ERa on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERa in breast cancer tissues.

WB, IHC-P, IHC-Fr

IHC-Fr 1: 1:40 - 1:60, IHC-P 2: 1:40 - 1:80, WB: 1:50 - 1:100

Human

Recombinant human estrogen receptor (alpha form).

Mouse

Monoclonal

6F11

IgG1

Tissue Culture Supernatant

Liquid

Supplied in 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human estrogen alpha antibody, clone 6F11 recognizes the human estrogen receptor alpha chain (ERa), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERa is a ~65 kDa steroid hormone receptor containing an N-terminal (AF-1) ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an (AF-2) domain. ERa binds to DNA as a homodimer (Klinge 2001) and can also form heterodimers with Estrogen receptor beta. The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors (Yaziji et al. 2008). Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy (Bhargava et al. 2012).Mouse anti human estrogen alpha antibody, clone 6F11 has been used successfully for identification of ERa on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERa in breast cancer tissues (Ambroise et al. 2011).

ER, ER-alpha, ESR, ESR1, Estradiol receptor, Estrogen receptor, NR3A1, Nuclear receptor subfamily 3 group A member 1