Mouse monoclonal [ER506] antibody to Estrogen Receptor alpha (CF®640R).
Applications
Flow Cytometry (Intracellular), IHC, IHC-P, WB
Positive Control
MCF-7 cells or breast cancers.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes.
Reactivity
Human
Immunogen
Recombinant human Estrogen Receptor alpha (ESR1) protein corresponding to amino acids 2-185.
Host
Mouse
Clonality
Monoclonal
Clone ID
ER506
Isotype
IgG1
Light Chains
kappa
Conjugate
CF®640R
Concentration
0.1 mg/mL
Predicted MW
67 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Synonyms
ER, ER-alpha, ESR, ESR1, Estradiol receptor, Estrogen receptor, NR3A1, Nuclear receptor subfamily 3 group A member 1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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