Recombinant mouse monoclonal [rESR1/1935] antibody to Estrogen Receptor alpha (CF®640R).
Applications
IHC, IHC-P, WB
Positive Control
MCF-7 cells or breast cancer tissue.
Dilutions
WB: 1-2 µg/mL, IHC-P: 1-2 µg/mL for 30 minutes at room temperature. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Recombinant full-length human Estrogen Receptor alpha (ESR1) protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
rESR1/1935
Isotype
IgG1
Conjugate
CF®640R
Concentration
0.1 mg/mL
Predicted MW
67 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Synonyms
ER, ER-alpha, ESR, ESR1, Estradiol receptor, Estrogen receptor, NR3A1, Nuclear receptor subfamily 3 group A member 1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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