Anti-FAK Antibody (A88000)

Name: Anti-FAK Antibody
Brand: Antibodies.com
SKU: A88000
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to FAK for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-FAK Antibody

Description

Rabbit polyclonal antibody to FAK.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 734-834 of human FAK (NP_722560.1).

Sequence

QHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

125 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

FADK 1, FAK1, Focal adhesion kinase 1, Focal adhesion kinase-related nonkinase, FRNK, p125FAK, pp125FAK, PPP1R71, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, PTK2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (9)

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Western Blot - Anti-FAK Antibody (A88000)

Western blot analysis of extracts of various cell lines, using Anti-FAK Antibody (A88000) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-FAK Antibody (A88000)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-FAK Antibody (A88000) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-FAK Antibody (A88000)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-FAK Antibody (A88000) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-FAK Antibody (A88000)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-FAK Antibody (A88000) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.