Anti-FHIP2B Antibody (A305971)

Name: Anti-FHIP2B Antibody
Brand: Antibodies.com
SKU: A305971

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This product is discontinued

Anti-FHIP2B Antibody (A305971) has been discontinued and is no longer available.

View all FHIP2B Antibodies.

Specifications

Name

Anti-FHIP2B Antibody

Description

Rabbit polyclonal antibody to FHIP2B.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 74-303 of human FAM160B2 (NP_073586.5).

Sequence

GEAGPCLEYLLQHKILETLCTLGKAEYPPGMRQQVFQFFSKVLAQVQHPLLHYLSVHRPVQKLLRLGGTASGSVTEKEEVQFTTVLCSKIQQDPELLAYILEGKKIVGRKKACGEPTALPKDTTSHGDKDCSHDGAPARPQLDGESCGAQALNSHMPAETEELDGGTTESNLITSLLGLCQSKKSRVALKAQENLLLLVSMASPAAATYLVQSSACCPAIVRHLCQLYRS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

82 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FAM160B2, FHF complex subunit HOOK-interacting protein 2B, Retinoic acid-induced protein 16

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-FHIP2B Antibody (A305971)

Western blot analysis of extracts of various cell lines, using Anti-FHIP2B Antibody (A305971) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunofluorescence - Anti-FHIP2B Antibody (A305971)

Immunofluorescence analysis of C6 cells using Anti-FHIP2B Antibody (A305971) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-FHIP2B Antibody (A305971)

Immunofluorescence analysis of NIH/3T3 cells using Anti-FHIP2B Antibody (A305971) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-FHIP2B Antibody (A305971)

Immunoprecipitation analysis of 600µg extracts of rat testis cells using 3µg of Anti-FHIP2B Antibody (A305971). This Western blot was performed on the immunoprecipitate using Anti-FHIP2B Antibody (A305971) at a dilution of 1:1000.