Anti-gamma Tubulin Antibody [TU-30] (A86254)

Name: Anti-gamma Tubulin Antibody [TU-30]
Brand: Antibodies.com
SKU: A86254
Price: 125.0 GBP
Availability: InStock

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$210

Mouse monoclonal [TU-30] antibody to gamma Tubulin for ICC, WB and Flow Cytometry (Intracellular).

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-gamma Tubulin Antibody [TU-30]

Description

Mouse monoclonal [TU-30] antibody to gamma Tubulin.

Specificity

This antibody recognizes the C terminus of human gamma Tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope is located in the amino acid sequence TRPDYI (a.a. 439-444 in human), which is present on human gamma Tubulin 1 but not on human gamma Tubulin 2.

Applications

ICC, WB, Flow Cytometry (Intracellular)

Dilutions

IHC: 1-2 µg/ml. Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive Control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells, WB: Recommended dilution 1-2 µg/ml; Reducing conditions.

Reactivity

Protozoa, Chicken, Bovine, Rat, Mouse, Porcine, Human, Plants

Immunogen

C-terminal peptide of gamma-tubulin counjugated to KLH.

Host

Mouse

Clonality

Monoclonal

Clone ID

TU-30

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Predicted MW

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Gamma-1-tubulin, Gamma-2-tubulin, Gamma-tubulin complex component 1, GCP-1, TUBG, TUBG1, TUBG2, Tubulin gamma-1 chain, Tubulin gamma-2 chain

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Anti-gamma Tubulin Antibody [TU-30] (A86254)

Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using Anti-gamma Tubulin Antibody [TU-30] (A86254), (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).

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Flow Cytometry - Anti-gamma Tubulin Antibody [TU-30] (A86254)

Separation of MCF-7 cells stained with Anti-gamma Tubulin Antibody [TU-30] (A86254), (concentration in sample 9 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

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Anti-gamma Tubulin Antibody [TU-30] (A86254)

Immunocytochemistry staining of murine fibroblasts using Anti-gamma Tubulin Antibody [TU-30] (A86254) direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).

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Anti-gamma Tubulin Antibody [TU-30] (A86254)

Immunocytochemistry staining of microtubular networks in 3T3 mouse fibroblasts.A - metaphase; B - anaphase; C - telophaseGamma-tubulin (red) stained using Anti-gamma Tubulin Antibody [TU-30] (A86254) , alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).