Anti-gamma Tubulin Antibody [TU-32] (A86251)

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$210

Mouse monoclonal [TU-32] antibody to gamma Tubulin for WB and ICC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-gamma Tubulin Antibody [TU-32]

Description

Mouse monoclonal [TU-32] antibody to gamma Tubulin.

Specificity

The antibody recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).

Applications

WB, ICC

Dilutions

WB: 1-2 µg/ml; Reducing conditions, IHC: Methanol/acetone fixation required.

Reactivity

Protozoa, Plants, Animals

Immunogen

Human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449.

Sequence

EYHAATRPDYISWGTQ

Host

Mouse

Clonality

Monoclonal

Clone ID

TU-32

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Predicted MW

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Gamma-1-tubulin, Gamma-2-tubulin, Gamma-tubulin complex component 1, GCP-1, TUBG, TUBG1, TUBG2, Tubulin gamma-1 chain, Tubulin gamma-2 chain

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Anti-gamma Tubulin Antibody [TU-32] (A86251)

Western blotting analysis of human gamma-tubulin using Anti-gamma Tubulin Antibody [TU-32] (A86251) on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-gamma-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for gamma-tubulin at approximately 46 kDa.

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Anti-gamma Tubulin Antibody [TU-32] (A86251)

Western blotting analysis of differential reactivity of monoclonal antibodies to ?-tubulin with human ?-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human ?-tubulin 1 (?-Tb1) or ?-tubulin 2 (?-Tb2), probed with ?-tubulin antibodies: Anti-gamma Tubulin Antibody [TU-30] (A86254) and Anti-gamma Tubulin Antibody [TU-32] (A86251), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of ?-Tb1 or ?-Tb2 probed with Abs to ?-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.

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