Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313)
Western blot analysis of extracts of various cell lines, using Anti-GATA3 Antibody (A307313) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-GATA3 Antibody [ARC0115] (A307313)
Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-GATA3 Antibody (A307313). This Western blot was performed on the immunoprecipitate using Anti-GATA3 Antibody (A307313) at a dilution of 1:500.
Chromatin immunoprecipitation (ChIP) analysis of extracts of MCF7 cells, using Anti-GATA3 Antibody (A307313) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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