Anti-GFAP Antibody (A270545)

Immunofluorescent analysis of mouse hippocampus section stained with Anti-GFAP Antibody (A270545), at a dilution of 1:5,000, in green, and co-stained with Anti-NeuN Antibody [1B7] (A85405), at a dilution of 1:2,000, in red. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. Anti-GFAP Antibody (A270545) stains the network of astrocytic glial cells, while Anti-NeuN Antibody [1B7] (A85405) specifically labels nuclei and proximal perikarya of neurons.

Western blot analysis of brain lysates from different species using Anti-GFAP Antibody (A270545), at a dilution of 1:5,000, in green. The lanes contain: [Lane 1] protein standard (red), [Lane 2] rat cortex, [Lane 3] rat cerebellum, [Lane 4] mouse cortex, [Lane 5] mouse cerebellum, [Lane 6] cow cortex, and [Lane 7] cow cerebellum. The strong band at ~50 kDa corresponds to GFAP protein. Smaller proteolytic fragments of GFAP are also detected on the blot.

Immunohistochemistry analysis of a formalin fixed paraffin embedded mouse cerebral cortex section with Anti-GFAP Antibody (A270545) at a dilution of 1:10,000 detected with DAB (brown) using the Vector Elite ABC-HRP detection and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). In normal brain tissue, the GFAP antibody specifically labels astrocytes. It can also detect chronically activated microglia in neurodegenerative conditions.