Anti-Ghrelin Antibody (A305137)

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This product is discontinued

Anti-Ghrelin Antibody (A305137) has been discontinued and is no longer available.

View all Ghrelin Antibodies.

Specifications

Name

Anti-Ghrelin Antibody

Description

Rabbit polyclonal antibody to Ghrelin.

Applications

WB, ICC/IF, IHC

Dilutions

WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50

Reactivity

Human, Rat

Immunogen

Synthetic peptide from the mid-protein of Human Ghrelin.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~13 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Appetite-regulating hormone, GHRL, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, MTLRP, Protein M46

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Immunohistochemistry - Anti-Ghrelin Antibody (A305137)

Immunohistochemistry analysis of human stomach, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Ghrelin Antibody (A305137) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

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Immunofluorescence - Anti-Ghrelin Antibody (A305137)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Ghrelin Antibody (A305137), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasmic. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ghrelin Antibody. (D) Composite.

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Western Blot - Anti-Ghrelin Antibody (A305137)

Western blot analysis of rat Pancreas showing detection of ~12.9 kDa Ghrelin protein using Anti-Ghrelin Antibody (A305137) at 1:1,000 for 60 minutes at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat Pancreas cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~12.9 kDa.

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Immunofluorescence - Anti-Ghrelin Antibody (A305137)

Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Ghrelin Antibody (A305137), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasmic. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Ghrelin Antibody. (D) Composite.