GOLPH2 expression in Mouse large intestine lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-GOLPH2 Antibody (A89951) at a dilution of 1:9000 and detected with chemiluminescence.
GOLPH2 expression in human colon carcinoma tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-GOLPH2 Antibody (A89951) at a dilution of 1:100.
GOLPH2 expression in MCF7 cells analyzed by immunofluorescence. Staining was performed with Anti-GOLPH2 Antibody (A89951) followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
GOLPH2 expression in Hela cells analyzed by immunofluorescence. Staining was performed with Anti-GOLPH2 Antibody (A89951) at a dilution of 1:200. Nuclei were stained with DAPI (blue).
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