Anti-GPA33 Antibody [ARC2060] (A308311)

Name: Anti-GPA33 Antibody [ARC2060]
Brand: Antibodies.com
SKU: A308311
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC2060] antibody to GPA33 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-GPA33 Antibody [ARC2060]

Description

Rabbit monoclonal [ARC2060] antibody to GPA33.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 220-319 of human GPA33 (Q99795).

Sequence

QFCNITVAVRSPSMNVALYVGIAVGVVAALIIIGIIIYCCCCRGKDDNTEDKEDARPNREAYEEPPEQLRELSREREEEDDYRQEEQRSTGRESPDHLDQ

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2060

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

39 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cell surface A33 antigen, Glycoprotein A33

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-GPA33 Antibody [ARC2060] (A308311)

Western blot analysis of extracts of various cell lines, using Anti-GPA33 Antibody [ARC2060] (A308311) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Immunohistochemistry - Anti-GPA33 Antibody [ARC2060] (A308311)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-GPA33 Antibody [ARC2060] (A308311) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-GPA33 Antibody [ARC2060] (A308311)

Immunofluorescence analysis of human colon carcinoma using Anti-GPA33 Antibody [ARC2060] (A308311) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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