Recombinant chimeric monoclonal [DMC497] antibody to GPA33.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit / Human
Clonality
Monoclonal
Clone ID
DMC497
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.
Flow cytometry analysis of Anti-GPA33 Antibody [DMC497] - BSA and Azide free (A318696) on Expi293 cells transfected with human GPA33 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Flow cytometry analysis of antigen binding of Anti-GPA33 Antibody [DMC497] - BSA and Azide free (A318696). (A) Anti-GPA33 Antibody [DMC497] - BSA and Azide free (A318696) does not bind to 293T cells that do not express GPA33. (B) A clear peak shift of Anti-GPA33 Antibody [DMC497] - BSA and Azide free (A318696) was seen compared to the control when incubated with GPA33-expressing HT55 cells, indicating strong binding of Anti-GPA33 Antibody [DMC497] - BSA and Azide free (A318696) to GPA33. Antibodies were incubated at 5 µg/ml.
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