Mouse monoclonal [1D9-M12] antibody to GPCR C5L2 (APC).
Specificity
This antibody recognizes an extracellular epitope on C5aR2 (C5L2), a C5a complement receptor, which is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
L1.2 cells transfected with human C5aR2.
Host
Mouse
Clonality
Monoclonal
Clone ID
1D9-M12
Isotype
IgG2a
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
Purified antibody is conjugated with APC under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling GPCR C5L2 with Anti-GPCR C5L2 Antibody [1D9-M12] (APC) (A320658), (10 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with GPCR C5L2
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-GPCR C5L2 Antibody [1D9-M12] (APC) (A320658)](https://cdn.antibodies.com/image/catalog/320/A320658_1.jpg?profile=product_top)
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![Flow Cytometry - Anti-GPCR C5L2 Antibody [1D9-M12] (APC) (A320658)](https://cdn.antibodies.com/image/catalog/320/A320658_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-GPCR C5L2 Antibody [1D9-M12] (APC) (A320658)](https://cdn.antibodies.com/image/catalog/320/A320658_1.jpg?profile=product_image)
![Flow Cytometry - Anti-GPCR C5L2 Antibody [1D9-M12] (APC) (A320658)](https://cdn.antibodies.com/image/catalog/320/A320658_2.jpg?profile=product_image)