This antibody recognizes an extracellular epitope on C5aR2 (C5L2), a C5a complement receptor, which is coexpressed with C5aR1 (CD88) in neutrophils, as well as i.e. in mast cells, astrocytes, or macrophages.
Applications
WB, Flow Cytometry
Dilutions
Flow Cytometry: 2-6 µg/ml.
Reactivity
Human, Non-Human Primates
Immunogen
L1.2 cells transfected with human C5aR2.
Host
Mouse
Clonality
Monoclonal
Clone ID
1D9-M12
Isotype
IgG2a
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of monocytes stained with Anti-C5AR2 Antibody [1D9-M12] (concentration in sample is 5 µg/ml, GAM-APC, red-filled) from monocytes unstained by primary antibody (GAM-APC, black-dashed) in flow cytometry analysis (surface staining).
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