This antibody targets the C-terminal domain of human GPCR C5L2, recognizing the non-phosphorylated form. It is optimized for Western blot analysis and facilitates immunoprecipitation from cell and tissue lysates.
Applications
WB, ICC
Dilutions
WB: 1:1,000, ICC: 1:200
Reactivity
Human
Immunogen
Synthetic peptide corresponding to C-terminal of human GPCR C5L2. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot validation of Complement C5a receptor 2 (GPCR C5L2) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing GPCR C5L2 were immunoblotted with the phosphorylation-independent anti-GPCR C5L2 antibody (A334504) at a 1:1000 dilution.
Immunocytochemical analysis of Complement C5a receptor 2 (GPCR C5L2) in transfected HEK293 cells. Native HEK293 cells (MOCK) or cells stably expressing GPCR C5L2 were stained with the phosphorylation-independent anti-GPCR C5L2 antibody (A334504) at a 1:200 dilution.
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