Rabbit polyclonal antibody to GSK3α / β (Phospho-Tyr279 / 216)
Specificity
The antibody detects endogenous level of GSK3α / β only when phosphorylated at tyrosine 279 / 216.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 279 / 216 (V-S-Y(p)-I-C) derived from Human GSK3α / β.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
46, 51kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3α/β (Phospho-Tyr279/216) Antibody #11301 (left) or the same antibody preincubated with blocking peptide #51301 (right).
