Anti-Histone H2A.X (phospho Thr142) Antibody (A320117)

Anti-Histone H2A.X (phospho Thr142) Antibody

Rabbit polyclonal antibody to Histone H2A.X (phospho Thr142).

This antibody recognises Histone H2A.X when phosphorylated at tyrosine 142.Histone H2A.X is a member of the histone H2A family, one of the four core histones making up the nucleosome core particle. Histone H2A variant H2A.X is a variant of histone H2A that regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein, to the sites of DNA damage. Phosphorylation of histone H2A.X at tyrosine 142 is implicated in a number of biological processes, including DNA repair. K-Ras-ERK1/2 down-regulates phosphorylation of H2A.X at the site of tyrosine 142 by increasing degradation of WSTF and contributes to the progression of gastric cancer. Wide species cross-reactivity is expected from Rabbit anti Human histone H2A.X (pTyr142) antibody based on sequence.

WB: 1:500 - 1:1,000

Human

A peptide containing phosphorylated tyrosine 142 of human histone H2A.X.

Rabbit

Polyclonal

IgG

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Human H2A.X (pTyr142) antibody recognizes histone H2A.X when phosphorylated at tyrosine 142.Histone H2A.X is a member of the histone H2A family, one of the four core histones making up the nucleosome core particle. Histone H2A variant H2A.X is a variant of histone H2A that regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein, to the sites of DNA damage. Phosphorylation of histone H2A.X at tyrosine 142 is implicated in a number of biological processes, including DNA repair. K-Ras-ERK1/2 down-regulates phosphorylation of H2A.X at the site of tyrosine 142 by increasing degradation of WSTF and contributes to the progression of gastric cancer (Dong et al. 2019).Wide species cross-reactivity is expected from Rabbit anti Human histone H2A.X (pTyr142) antibody based on sequence.

H2a/x, H2AFX, H2AX, Histone H2AX