Anti-Histone H2A.Z Antibody [ARC1048] (A306893)

Name: Anti-Histone H2A.Z Antibody [ARC1048]
Brand: Antibodies.com
SKU: A306893
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1048] antibody to Histone H2A.Z for WB, IP and ChIP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Knockout Validated Antibody Nuclear Marker

Specifications

Name

Anti-Histone H2A.Z Antibody [ARC1048]

Description

Rabbit monoclonal [ARC1048] antibody to Histone H2A.Z.

Applications

WB, IP, ChIP

Dilutions

WB: 1:500-1:1,000, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2A.Z (P0C0S5).

Sequence

MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1048

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

H2A/z, H2AFZ, H2AZ, H2AZ1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Histone H2A.Z Antibody [ARC1048] (A306893)

Western blot analysis of extracts of various cell lines, using Anti-Histone H2A.Z Antibody [ARC1048] (A306893) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-Histone H2A.Z Antibody [ARC1048] (A306893)

Western blot analysis of extracts from wild type(WT) and Histone H2A. Z knockout (KO) 293T(KO) cells, using Anti-Histone H2A.Z Antibody [ARC1048] (A306893) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Chromatin Immunoprecipitation - Anti-Histone H2A.Z Antibody [ARC1048] (A306893)

Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-Histone H2A.Z Antibody [ARC1048] (A306893) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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