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Anti-Histone H2AX (phospho S139) Antibody (A12470)

Western Blot - Anti-Histone H2AX (phospho S139) Antibody (AP0245) - Antibodies.com
This product is discontinued

Anti-Histone H2AX (phospho S139) Antibody (A12470) has been discontinued and is no longer available.

View all Histone H2AX Antibodies.

  • Specifications

Name
Anti-Histone H2AX (phospho S139) Antibody
Description
Mouse polyclonal antibody to Histone H2AX (phospho S139).
Applications
WB, IF
Dilutions
WB: 1:500-1:2000, IF: 1:100-1:200
Reactivity
Human, Mouse
Immunogen
A phospho specific peptide corresponding to residues surrounding S139 of human H2AFX.
Host
Mouse
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
17kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
H2a/x, H2AFX, H2AX
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Histone H2AX (phospho S139) Antibody (AP0245) - Antibodies.comEnlarge Image

Western Blot - Anti-Histone H2AX (phospho S139) Antibody (A12470)

Western blot analysis of extracts of various cells, using Anti-Histone H2AX (phospho S139) Antibody (AP0245) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

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Proteins predicted to interact with Histone H2A.X

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.3% Relevancy Score
98.8% Relevancy Score
98.5% Relevancy Score
98.3% Relevancy Score
98% Relevancy Score