Anti-Histone H3.1 Antibody [1D4F2] (A320270)

Anti-Histone H3.1 Antibody [1D4F2]

Mouse monoclonal [1D4F2] antibody to Histone H3.1.

This antibody recognises Histone H3 variant H3.1.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). In contrast to histone H3.3, incorporation of histone H3.1 and H3.2 into nucleosomes is replication dependent rather than occurring throughout the cell cycle.

IF, WB

IF: 0.4 µg/ml - 2 µg/ml, WB: 0.2 µg/ml - 2 µg/ml

Human, Broad

A peptide corresponding to amino acids 21-39 of human histone H3.1. This region is 100% identical in human histone H3.2.

LATKAARKSAPATGGVKKP

Mouse

Monoclonal

1D4F2

IgG2b

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human histone H3.1 antibody, clone 1D4F2 recognizes histone H3 variant H3.1.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). In contrast to histone H3.3, incorporation of histone H3.1 and H3.2 into nucleosomes is replication dependent rather than occurring throughout the cell cycle (Tagami et al. 2004).

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o