Anti-Histone H3.1 (phospho Ser28) Antibody [5D10D4] (A320278)

Anti-Histone H3.1 (phospho Ser28) Antibody [5D10D4]

Rat monoclonal [5D10D4] antibody to Histone H3.1 (phospho Ser28).

This antibody recognises Histone H3.1 when phosphorylated at serine 28.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). In contrast to histone H3.3, incorporation of histone H3.1 and histone H3.2 into nucleosomes is replication dependent rather than occurring throughout the cell cycle. Phosphorylation of histone H3.1 at serine 28 occurs in early mitosis and during premature chromosome condensation in cells that are in S-phase of the cell cycle. Rat anti Human histone H3.1 (pSer28) antibody, clone 5D10D4 does not react with histone H3.3 phosphorylated on serine 28. Wide species cross-reactivity is expected based on sequence.

IF, WB

IF: 2 µg/ml - 10 µg/ml, WB: 0.5 µg/ml - 2 µg/ml

Human, Broad

A peptide containing phosphorylated serine 28 of human histone H3.1.

Rat

Monoclonal

5D10D4

IgG2b

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Human histone H3.1 (pSer28) antibody, clone 5D10D4 recognizes Histone H3.1 when phosphorylated at serine 28.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). In contrast to histone H3.3, incorporation of histone H3.1 and histone H3.2 into nucleosomes is replication dependent rather than occurring throughout the cell cycle (Tagami et al. 2004). Phosphorylation of histone H3.1 at serine 28 occurs in early mitosis and during premature chromosome condensation in cells that are in S-phase of the cell cycle (Zhong et al. 2003).Rat anti Human histone H3.1 (pSer28) antibody, clone 5D10D4 does not react with histone H3.3 phosphorylated on serine 28. Wide species cross-reactivity is expected based on sequence.

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o