Anti-Histone H3 (acetyl Lys 9) Antibody [2G1F9] (A320273)

Anti-Histone H3 (acetyl Lys 9) Antibody [2G1F9]

Rat monoclonal [2G1F9] antibody to Histone H3 (acetyl Lys 9).

This antibody recognises Histone H3 when acetylated at lysine 9.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of histone H3 at lysine 4 usually leads to the transcriptional activation of genes. Wide species cross-reactivity is expected from Rat anti Human histone H3 (Ac9) antibody based on sequence.

IF, WB, ChIP

ChIP: 5 µg per ChIP - 10 µg per ChIP, IF: 1 µg/ml - 2 µg/ml, WB: 0.5 µg/ml - 2 µg/ml

Human, Broad

A peptide containing acetyl-lysine 9 of human histone H3.

Rat

Monoclonal

2G1F9

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Human histone H3 (Ac9) antibody, clone 2G1F9 recognizes histone H3 when acetylated at lysine 9.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Hyperacetylation of histone tails makes chromatin more accessible to DNA-binding proteins by weakening histone-DNA and nucleosome-nucleosome interactions (Kuo and Allis, 1998). Acetylation of histone H3 at lysine 4 usually leads to the transcriptional activation of genes (Hazzlin and Mahadevan, 2005).Wide species cross-reactivity is expected from Rat anti Human histone H3 (Ac9) antibody based on sequence.

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o