Anti-Histone H3 (di methyl Lys79) Antibody (A320136)

Anti-Histone H3 (di methyl Lys79) Antibody

Rabbit polyclonal antibody to Histone H3 (di methyl Lys79).

This antibody recognises Histone H3 when di-methylated at lysine 79.Histone H3 is one of the four core histones that make up the nucleosome core particle. Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones can be mono-, di- or tri-methylated by histone methyltransferases. Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Methylation events that weaken the binding between histone tails and DNA lead to increased transcription because they make the DNA more accessible to transcription factor proteins and RNA polymerase. Methylation of histones is therefore crucial for the regulation of gene expression. Lysine 79 of histone H3 may be mono-, di- or tri-methylated by the methylase DOT1. Methylation of lysine 79 of histone H3 acts as a marker of inactive chromatin regions that is essential for silencing of transcription of proteins such as SIR3. Wide species cross-reactivity is expected from Rabbit anti Human di-methyl-histone H3 (Lys79) antibody based on sequence.

ChIP, WB

ChIP: 10 µg per ChIP, WB: 1 µg/ml - 2 µg/ml

Human, Broad

A peptide including di-methylated lysine 79 of histone H3.

Rabbit

Polyclonal

IgG

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Human di-methyl-histone H3 (Lys79) antibody recognizes histone H3 when di-methylated at lysine 79.Histone H3 is one of the four core histones that make up the nucleosome core particle. Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones can be mono-, di- or tri-methylated by histone methyltransferases. Depending on which amino acid residues are methylated, methylation of histones may increase or decrease the transcription of genes. Methylation events that weaken the binding between histone tails and DNA lead to increased transcription because they make the DNA more accessible to transcription factor proteins and RNA polymerase. Methylation of histones is therefore crucial for the regulation of gene expression (Gupta et al. 2010). Lysine 79 of histone H3 may be mono-, di- or tri-methylated by the methylase DOT1. Methylation of lysine 79 of histone H3 acts as a marker of inactive chromatin regions that is essential for silencing of transcription of proteins such as SIR3 (Ng et al. 2003).Wide species cross-reactivity is expected from Rabbit anti Human di-methyl-histone H3 (Lys79) antibody based on sequence.

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o