Anti-Histone H3 (phospho Ser10) Antibody [6G8B7] (A320281)

Anti-Histone H3 (phospho Ser10) Antibody [6G8B7]

Rat monoclonal [6G8B7] antibody to Histone H3 (phospho Ser10).

This antibody recognises Histone H3 when phosphorylated at serine 10.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation. Phosphorylation of histone H3 has been shown to occur during the early stages of mitosis when chromosomes begin to condense, and during premature condensing of chromosomes during S-phase of the cell cycle. Phosphorylation of histone H3 at serine 10 has been linked to both transcription and cell division. Broad species cross-reactivity is expected from Rat anti Human histone H3 (pSer10) antibody based on sequence.

IF, WB, ELISA

IF: 0.5 µg/ml - 2 µg/ml, WB: 0.2 µg/ml - 2 µg/ml

Human, Broad

A peptide corresponding to amino acids 1-19 including phosphorylated serine 10 of human histone H3.

MARTKQTARKSTGGKAPRK

Rat

Monoclonal

6G8B7

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Human histone H3 (pSer10) antibody, clone 6G8B7 recognizes histone H3 when phosphorylated at serine 10.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation (Koch et al. 2007). Phosphorylation of histone H3 has been shown to occur during the early stages of mitosis when chromosomes begin to condense, and during premature condensing of chromosomes during S-phase of the cell cycle. Phosphorylation of histone H3 at serine 10 has been linked to both transcription and cell division (Hans and Dimitrov, 2001).Broad species cross-reactivity is expected from Rat anti Human histone H3 (pSer10) antibody based on sequence.

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o