Anti-Histone H3 (phospho Thr32) Antibody [6C7G12] (A320280)

Anti-Histone H3 (phospho Thr32) Antibody [6C7G12]

Rat monoclonal [6C7G12] antibody to Histone H3 (phospho Thr32).

This antibody recognises Histone H3 when phosphorylated at threonine 32.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation. Phosphorylation of histone H3 at threonine 32 has been shown to be enhanced in cells undergoing mitosis and is thought to play a distinct role due to its unique distribution across chromosomes compared to other histone H3 phosphorylation events. Wide species cross-reactivity is expected from Rat anti Human histone H3 (pThr32) antibody based on sequence.

WB: 1 µg/ml - 2 µg/ml

Human, Broad

A peptide containing phosphorylated threonine 32 of human histone H3.1.

Rat

Monoclonal

6C7G12

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Human histone H3 (pThr32) antibody, clone 6C7G12 recognizes histone H3 when phosphorylated at threonine 32.Histone H3 is one of the four core histones that make up the nucleosome core particle. Nucleosomes are the smallest subunit of chromatin and are made up of 146 bp of DNA wrapped around an octamer comprised of pairs of the four core histones (H2A, H2B, H3, and H4) (Smith, 1991). Histones may be modified in several ways including acetylation, methylation, phosphorylation, ubiquitination, glycosylation, and SUMOylation. Phosphorylation of histone H3 at threonine 32 has been shown to be enhanced in cells undergoing mitosis and is thought to play a distinct role due to its unique distribution across chromosomes compared to other histone H3 phosphorylation events (Hans and Dimitrov, 2001).Wide species cross-reactivity is expected from Rat anti Human histone H3 (pThr32) antibody based on sequence.

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o