This antibody recognizes an extracellular epitope of HLA F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.
Applications
Flow Cytometry, WB
Dilutions
Flow Cytometry: 1-5 µg/ml; Extracellular and Intracellular staining.
Reactivity
Human
Immunogen
Inclusion body-derived HLA F heavy chain.
Host
Mouse
Clonality
Monoclonal
Clone ID
3D11
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CDA12, HLA 5.4, HLA class I histocompatibility antigen, alpha chain F, HLA class I molecule, HLA F antigen, HLA-CDA12, HLA-F, HLAF, HLAF_HUMAN, Leukocyte antigen F, MHC class I antigen F
Flow Cytometry - Anti-HLA F Antibody [3D11] (A121802)
Separation of human activated lymphocytes stained with Anti-HLA F Antibody [3D11] (A121802), (concentration in sample 5 µg/ml, GAM APC, red-filled) from human activated lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of human PMA + Ionomycin stimulated peripheral blood mononuclear cells.
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