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Anti-HLA F Antibody [3D11] (PE) (A121831)

Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831) Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831) Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831) Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831)
$210
Product Datasheet

Mouse monoclonal [3D11] antibody to HLA F (PE) for Flow Cytometry.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-HLA F Antibody [3D11] (PE)
Description
Mouse monoclonal [3D11] antibody to HLA F (PE).
Specificity
This antibody recognizes an extracellular epitope of HLA F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-5 µg/ml; Extracellular and Intracellular staining.
Reactivity
Human
Immunogen
Inclusion body-derived HLA F heavy chain.
Host
Mouse
Clonality
Monoclonal
Clone ID
3D11
Isotype
IgG1
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Predicted MW
42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CDA12, HLA 5.4, HLA class I histocompatibility antigen, alpha chain F, HLA class I molecule, HLA F antigen, HLA-CDA12, HLA-F, HLAF, HLAF_HUMAN, Leukocyte antigen F, MHC class I antigen F
Isotype Controls
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831)Enlarge Image

Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831)

Flow cytometry multicolor intracellular staining pattern of human lymphocytes stained using Anti-CD20 Antibody [2H7] (PE) (A85692), (10 µl reagent / 100 µl of peripheral whole blood) and Anti-HLA F Antibody [3D11] (PE) (A121831), (concentration in sample 5 µg/ml, red) or Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 5 µg/ml, same as A121831 concentration, black).
Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831)Enlarge Image

Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831)

Flow cytometry multicolor surface staining of human lymphocytes stained using Anti-CD20 Antibody [2H7] (PE) (A85692), (10 µl reagent / 100 µl of peripheral whole blood) and Anti-HLA F Antibody [3D11] (PE) (A121831), (concentration in sample 5 µg/ml, red) or Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 5 µg/ml, same as anti-HLA-F PE concentration, black).
Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831)Enlarge Image

Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831)

Separation of lymphocytes stained with Anti-HLA F Antibody [3D11] (PE) (A121831), (concentration in sample 5 µg/ml, red-filled) from lymphocytes stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 5 µg/ml, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of human stimulated (PMA + Ionomycin) whole blood cells.
Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831)Enlarge Image

Flow Cytometry - Anti-HLA F Antibody [3D11] (PE) (A121831)

Separation of lymphocytes stained with Anti-HLA F Antibody [3D11] (PE) (A121831), (concentration in sample 5 µg/ml, red-filled) from lymphocytes stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 5 µg/ml, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (PMA + Ionomycin) whole blood cells.
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