Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein R, HMG-I(Y), HMGIY
Western blot analysis of extracts of various cell lines, using Anti-HMGA1 Antibody (A12693) at 1:3000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
