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Anti-HMGB1 Antibody (A12694)

  • Specifications

Name
Anti-HMGB1 Antibody
Description
Rabbit polyclonal antibody to HMGB1.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide of human HMGB1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
25kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
High mobility group protein 1, High mobility group protein B1, HMG-1, HMG1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (10)

Western Blot - Anti-HMGB1 Antibody (A0719) - Antibodies.comEnlarge Image

Western Blot - Anti-HMGB1 Antibody (A12694)

Western blot analysis of extracts of various cell lines, using Anti-HMGB1 Antibody (A0719) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Immunohistochemistry - Anti-HMGB1 Antibody (A0719) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-HMGB1 Antibody (A12694)

Immunohistochemistry of paraffin-embedded human breast cancer using Anti-HMGB1 Antibody (A0719) at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-HMGB1 Antibody (A0719) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-HMGB1 Antibody (A12694)

Immunohistochemistry of paraffin-embedded human colon using Anti-HMGB1 Antibody (A0719) at dilution of 1:100 (40x lens).
Immunohistochemistry - Anti-HMGB1 Antibody (A0719) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-HMGB1 Antibody (A12694)

Immunohistochemistry of paraffin-embedded mouse brain using Anti-HMGB1 Antibody (A0719) at dilution of 1:100 (40x lens).

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Proteins predicted to interact with HMGB1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.7% Relevancy Score
99.7% Relevancy Score
99.1% Relevancy Score
99.1% Relevancy Score
98.4% Relevancy Score