Western Blot - Anti-hnRNP K Antibody [ARC0512] (A307298)
Western blot analysis of extracts of various cell lines, using Anti-hnRNP K Antibody [ARC0512] (A307298) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-hnRNP K Antibody [ARC0512] (A307298)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-hnRNP K Antibody [ARC0512] (A307298) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-hnRNP K Antibody [ARC0512] (A307298)
Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-hnRNP K Antibody [ARC0512] (A307298) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-hnRNP K Antibody [ARC0512] (A307298)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-hnRNP K Antibody [ARC0512] (A307298) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-hnRNP K Antibody [ARC0512] (A307298)
Confocal imaging of U-2 0S cells using Anti-hnRNP K Antibody [ARC0512] (A307298), at a dilution of 1:100, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.