Rabbit polyclonal antibody to hnRNP K (Phospho-Ser216)
Specificity
The antibody detects endogenous levels of hnRNP K only when phosphorylated at serine 216.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 216 (S-E-S(p)-P-I) derived from Human hnRNP K.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Jurka cells, using hnRNP K (Phospho-Ser216) antibody #12140. The lane on the right is treated with the synthesized peptide.
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