This antibody recognises both denatured and native huntingtin in human brain. The combined use of Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051) and HDB4E10 (MCA2050) demonstrate that huntingtin is enriched in neuronal cells in the brain.
Applications
WB, IHC-Fr, IP
Reactivity
Mouse, Rabbit, Human
Immunogen
Recombinant protein corresponding to amino acids 2703 - 2911 of huntingtin.
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Huntingtin antibody, clone HDC8A4 reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein.Mouse anti Huntingtin antibody, clone HDC8A4 detects a ~350 kDa band on western blots but also detects smaller degradation products of huntingtin. Clone HDC8A4 recognizes both denatured and native huntingtin in human brain. The combined use of Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051) and HDB4E10 (MCA2050) demonstrate that huntingtin is enriched in neuronal cells in the brain.
Synonyms
HD, HD protein, HTT, Huntington disease protein, IT15
