Mouse monoclonal [MEM-140] antibody to IL-2 Receptor alpha.
Specificity
This antibody reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Applications
Flow Cytometry, IP
Dilutions
IP: Excellent for immunoisolation of CD25+ cells, Flow Cytometry: 1 µg/ml
Reactivity
Human
Immunogen
PHA-activated peripheral blood leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-140
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.