Mouse monoclonal [MEM-140] antibody to IL-2 Receptor alpha (Biotin).
Specificity
This antibody reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Applications
IP, Flow Cytometry
Dilutions
Flow Cytometry: 1 µg/ml
Reactivity
Human
Immunogen
PHA-activated peripheral blood leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-140
Isotype
IgM
Conjugate
Biotin
Purification
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentration
1 mg/ml
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store at 2-8°C. Do not freeze!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Surface staining of CD25 in PHA activated PBMC with Anti-CD25 Antibody (A85747).
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