Western blot analysis of extracts of various cell lines, using Anti-IL-25 Antibody (A16129) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
Immunofluorescence analysis of human lung cancer cells using Anti-IL-25 Antibody (A16129) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of rat bronchus cells using Anti-IL-25 Antibody (A16129) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of mouse lung cells using Anti-IL-25 Antibody (A16129) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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