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Anti-IL-7R (phospho Tyr449) Antibody (A93999)

Immunofluorescence - Anti-IL-7R (phospho Tyr449) Antibody (A1065) - Antibodies.com Western Blot - Anti-IL-7R (phospho Tyr449) Antibody (A1065) - Antibodies.com
This product is discontinued

Anti-IL-7R (phospho Tyr449) Antibody (A93999) has been discontinued and is no longer available.

View all CD127 Antibodies.

  • Specifications

Name
Anti-IL-7R (phospho Tyr449) Antibody
Description
Rabbit polyclonal antibody to IL-7R (phospho Tyr449).
Specificity
This antibody detects endogenous levels of IL-7R/CD127 only when phosphorylated at Tyr449.
Applications
WB, IF, ELISA
Dilutions
WB: 1:500~1:1000, ELISA: 1:5000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449 (amino acids 410-459).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
51kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CDw127, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, Interleukin-7 receptor subunit alpha
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Immunofluorescence - Anti-IL-7R (phospho Tyr449) Antibody (A1065) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-IL-7R (phospho Tyr449) Antibody (A93999)

Immunofluorescence analysis of HUVEC cells using Anti-IL-7R (phospho Tyr449) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-IL-7R (phospho Tyr449) Antibody (A1065) - Antibodies.comEnlarge Image

Western Blot - Anti-IL-7R (phospho Tyr449) Antibody (A93999)

Western blot analysis of lysates from COLO205 cells using Anti-IL-7R (phospho Tyr449) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Proteins predicted to interact with CD127

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.4% Relevancy Score
98.8% Relevancy Score
98.5% Relevancy Score
97.8% Relevancy Score
96% Relevancy Score