Mouse monoclonal [17C6] antibody to Integrin alpha 3 (FITC).
Specificity
This antibody recognises CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c. Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line.
Applications
Flow Cytometry
Dilutions
Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human
Immunogen
CD49c transfected cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
17C6
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD49c antibody, clone 17C6 recognizes CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c.Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line.
Synonyms
CD49 antigen-like family member C, CD49c, FRP-2, Galactoprotein B3, GAPB3, Integrin alpha-3, ITGA3, MSK18, VLA-3 subunit alpha