Mouse monoclonal [17C6] antibody to Integrin alpha 3 (PE).
Specificity
This antibody recognises CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c. Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human
Immunogen
CD49c transfected cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
17C6
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD49c antibody, clone 17C6 recognizes CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c.Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line.
Synonyms
CD49 antigen-like family member C, CD49c, FRP-2, Galactoprotein B3, GAPB3, Integrin alpha-3, ITGA3, MSK18, VLA-3 subunit alpha
Figure A. Alexa Fluor 647 conjugated Mouse anti Human EGFR and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Alexa Fluor 647 conjugated Mouse anti Human EGFR and Anti-Integrin alpha 3 Antibody [17C6] (PE) (A279054). All experiments performed on human peripheral blood gated on live single cells, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer
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