Mouse monoclonal [ASC-1] antibody to Integrin alpha 3 (APC).
Specificity
This antibody recognizes CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human SSC-9 cell line (squamous cell carcinoma).
Host
Mouse
Clonality
Monoclonal
Clone ID
ASC-1
Isotype
IgG1
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
125 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD49 antigen-like family member C, CD49c, FRP-2, Galactoprotein B3, GAPB3, Integrin alpha-3, ITGA3, MSK18, VLA-3 subunit alpha
Separation of HeLa cells stained with Anti-Integrin alpha 3 Antibody [ASC-1] (APC) (A320762), (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from HeLa cells stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (APC) (A86198), (concentration in sample 1.67 µg/ml, same as CD49c APC concentration, black-dashed) in flow cytometry analysis (surface staining).
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Proteins predicted to interact with Integrin alpha 3
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-Integrin alpha 3 Antibody [ASC-1] (APC) (A320762)](https://cdn.antibodies.com/image/catalog/320/A320762_1.jpg?profile=product_top)
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![Flow Cytometry - Anti-Integrin alpha 3 Antibody [ASC-1] (APC) (A320762)](https://cdn.antibodies.com/image/catalog/320/A320762_1.jpg?profile=product_image)