Western Blot - Anti-IRF3 (phospho Ser386) Antibody (A91788)
IRF3 expression in various lysates analyzed by western blot. Cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes following serum starvation overnight. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-IRF3 (Phospho S386) Antibody (A91788) at a dilution of 1:500 and detected with chemiluminescence.
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