Mouse monoclonal [MKI67/2466] antibody to Ki67 (Biotin).
Applications
Flow Cytometry (Intracellular), IF, IHC, IHC-P
Positive Control
Actively proliferating cells; skin, tonsil, or lymph node tissue.
Reactivity
Human
Immunogen
Recombinant fragment of human Ki67 protein corresponding to approximately amino acids 2293-2478 (exact sequence is proprietary).
Host
Mouse
Clonality
Monoclonal
Clone ID
MKI67/2466
Isotype
IgG2b
Light Chains
kappa
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
345 kDa and 395 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Synonyms
Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67, Proliferation marker protein Ki-67
Immunofluorescence of PFA-fixed HeLa cells using unconjugated Anti-Ki67 Antibody [MKI67/2466] followed by Goat Anti-Mouse IgG H&L Antibody (CF®488A) (A343429). Actin filaments were counterstained with CF®633 Phalloidin (magenta).
Immunofluorescence of human tonsil tissue FFPE section stained with unconjugated Anti-Ki67 Antibody [MKI67/2466] (A249348) followed by a goat anti-mouse HRP secondary antibody detected using CF®488A tyramide (green) prepared in Tyramide Amplification Buffer Plus.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-Ki67 Antibody [MKI67/2466] (CF®488A) (Blue). Fluorescence was detected in the FITC channel. Isotype control (red) is shown for comparison.
The protein microarray results shown are representative of the unconjugated form of the Anti-Ki67 Antibody [MKI67/2466] (Biotin). Protein microarray analysis of more than 19,000 full-length human proteins was performed using the Anti-Ki67 Antibody [MKI67/2466] (Biotin). The Z-score reflects antibody binding intensity to each protein on the HuProt™ array and is expressed as the number of standard deviations above the mean array signal. The S-score represents the difference in Z-score between adjacent ranked proteins and provides a measure of target specificity. Antibodies with an S-score of at least 2.5 are considered highly specific for their intended target.
Analysis of the unconjugated form of Anti-Ki67 Antibody [MKI67/2466]. Protein integrity and purity were assessed by SDS–PAGE under reducing conditions.
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_1.jpeg?profile=product_top)
![Immunofluorescence - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_2.jpg?profile=product_top)
![Immunofluorescence - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_3.jpg?profile=product_top)
![Flow cytometry - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_4.jpg?profile=product_top)
![Protein Array - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_5.jpg?profile=product_top)
![SDS-PAGE - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_6.jpg?profile=product_top)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_1.jpeg?profile=product_top_thumb)
![Immunofluorescence - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_2.jpg?profile=product_top_thumb)
![Immunofluorescence - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_3.jpg?profile=product_top_thumb)
![Flow cytometry - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_4.jpg?profile=product_top_thumb)
![Protein Array - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_5.jpg?profile=product_top_thumb)
![SDS-PAGE - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_6.jpg?profile=product_top_thumb)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_1.jpeg?profile=product_image)
![Immunofluorescence - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_2.jpg?profile=product_image)
![Immunofluorescence - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_3.jpg?profile=product_image)
![Flow cytometry - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_4.jpg?profile=product_image)
![Protein Array - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_5.jpg?profile=product_image)
![SDS-PAGE - Anti-Ki67 Antibody [MKI67/2466] (Biotin) (A354012) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354012_6.jpg?profile=product_image)
![Immunofluorescence - Anti-Ki67 Antibody [MKI67/2462] (Biotin) (A353988) - Antibodies.com](https://cdn.antibodies.com/image/catalog/353/A353988_1.jpg?profile=product_alternative)
![Immunofluorescence - Anti-Ki67 Antibody [MKI67/2465] (Biotin) (A354004) - Antibodies.com](https://cdn.antibodies.com/image/catalog/354/A354004_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Ki67 Antibody [MKI67/2465] (Biotin) (A252526) - Antibodies.com](https://cdn.antibodies.com/image/catalog/251/A251138_1.jpg?profile=product_alternative)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-Ki67 Antibody [MKI67/2461] (Biotin) (A353980) - Antibodies.com](https://cdn.antibodies.com/image/catalog/353/A353980_1.jpeg?profile=product_alternative)
![Immunohistochemistry (formalin-fixed, paraffin-embedded) - Anti-Ki67 Antibody [MKI67/2463] (Biotin) (A353996) - Antibodies.com](https://cdn.antibodies.com/image/catalog/353/A353996_1.jpeg?profile=product_alternative)
