Anti-KIF3A Antibody (A15313)

Name: Anti-KIF3A Antibody
Brand: Antibodies.com
SKU: A15313
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to KIF3A for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-KIF3A Antibody

Description

Rabbit polyclonal antibody to KIF3A.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3).

Sequence

RKALETKLDMEEEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPVPDKKEKDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKTGRRKRSAKPETVIDSLLQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

KIF3, Kinesin-like protein KIF3A, Microtubule plus end-directed kinesin motor 3A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-KIF3A Antibody (A15313)

Western blot analysis of extracts of various cell lines, using Anti-KIF3A Antibody (A15313) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-KIF3A Antibody (A15313)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-KIF3A Antibody (A15313) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-KIF3A Antibody (A15313)

Immunofluorescence analysis of MCF7 cells using Anti-KIF3A Antibody (A15313). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-KIF3A Antibody (A15313)

Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg of Anti-KIF3A Antibody (A15313). This Western blot was performed on the immunoprecipitate using Anti-KIF3A Antibody (A15313) at a dilution of 1:1000.

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