Western blot analysis of extracts of various cell lines, using Anti-Lamin B1 Antibody (A91106) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts from normal (control) and Lamin B1 knockout (KO) HeLa cells, using Anti-Lamin B1 Antibody (A91106) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
![Immunohistochemistry - Anti-Lamin B1 Antibody [119D5-F1] (A115608) - Antibodies.com](https://cdn.antibodies.com/image/catalog/115/A115608_1.jpg?profile=product_alternative)
