Anti-Laminin gamma 1 Antibody (A88836)

Name: Anti-Laminin gamma 1 Antibody
Brand: Antibodies.com
SKU: A88836
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to Laminin gamma 1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Laminin gamma 1 Antibody

Description

Rabbit polyclonal antibody to Laminin gamma 1.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1300-1609 of human LAMC1 (NP_002284.3).

Sequence

IDQKLKDYEDLREDMRGKELEVKNLLEKGKTEQQTADQLLARADAAKALAEEAAKKGRDTLQEANDILNNLKDFDRRVNDNKTAAEEALRKIPAINQTITEANEKTREAQQALGSAAADATEAKNKAHEAERIASAVQKNATSTKAEAERTFAEVTDLDNEVNNMLKQLQEAEKELKRKQDDADQDMMMAGMASQAAQEAEINARKAKNSVTSLLSIINDLLEQLGQLDTVDLNKLNEIEGTLNKAKDEMKVSDLDRKVSDLENEAKKQEAAIMDYNRDIEEIMKDIRNLEDIRKTLPSGCFNTPSIEKP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

LAMB2, LAMC1, Laminin B2 chain, Laminin subunit gamma-1, Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Laminin gamma 1 Antibody (A88836)

Western blot analysis of extracts of various cell lines, using Anti-Laminin gamma 1 Antibody (A88836) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-Laminin gamma 1 Antibody (A88836)

Western blot analysis of extracts of LO2 cells, using Anti-Laminin gamma 1 Antibody (A88836) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.

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Immunohistochemistry - Anti-Laminin gamma 1 Antibody (A88836)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Laminin gamma 1 Antibody (A88836) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Laminin gamma 1 Antibody (A88836)

Immunohistochemistry analysis of paraffin-embedded human uterus using Anti-Laminin gamma 1 Antibody (A88836) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.