This antibody recognizes an extracellular/luminal epitope of CD207, a transmembrane protein selectively expressed by Langerhans cells and by some other dendritic cell subsets. This antibody clone can target vaccine-peptides to dendritic cells.
Applications
ICC, Flow Cytometry, ELISA, IHC-Fr
Dilutions
Flow Cytometry: 0.5-4 µg/ml; Extracellular and Intracellular staining.
Reactivity
Human, Non-Human Primates
Immunogen
Fusion protein of human CD207 extracellular part and IgG Fc fragment.
Host
Mouse
Clonality
Monoclonal
Clone ID
2G3
Isotype
IgG1
Light Chains
lambda
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
C-type lectin domain family 4 member K, CD207, CLEC4K
Flow cytometry multicolor intracellular staining pattern of human stimulated (GM-CSF + IL-4 + TGF-beta) monocytes using Anti-Langerin Antibody [2G3] (A320770), (concentration in sample 0.56 µg/ml, GAM APC) and Anti-CD64 Antibody [10.1] (Pacific Blue), (4 µl reagent per milion cells in 100 µl of cell suspension).