This antibody recognizes an extracellular/luminal epitope of CD207, a transmembrane protein selectively expressed by Langerhans cells and by some other dendritic cell subsets. This antibody clone can target vaccine-peptides to dendritic cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
Reactivity
Human, Non-Human Primates
Immunogen
Fusion protein of human CD207 extracellular part and IgG Fc fragment.
Host
Mouse
Clonality
Monoclonal
Clone ID
2G3
Isotype
IgG1
Light Chains
lambda
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Purified antibody is conjugated with PE under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
C-type lectin domain family 4 member K, CD207, CLEC4K
Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells labeling Langerin with Anti-Langerin Antibody [2G3] (PE) (A320670), (10 µl reagent per milion cells in 100 µl of cell suspension).
Flow cytometry multicolor surface staining of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using Anti-Langerin Antibody [2G3] (PE) (A320670), (10 µl reagent per milion cells in 100 µl of cell suspension) and Anti-CD11c Antibody [BU15] (APC) (A86670), (10 µl reagent per milion cells in 100 µl of cell suspension).
Separation of human CD207 positive CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4), (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry surface staining of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells, labeling Langerin with Anti-Langerin Antibody [2G3] (PE) (A320670), (10 µl reagent per milion cells in 100 µl of cell suspension).
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Proteins predicted to interact with Langerin
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
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