Anti-LAT Antibody [LAT-01] (A86328)

Name: Anti-LAT Antibody [LAT-01]
Brand: Antibodies.com
SKU: A86328
Price: 125.0 GBP
Availability: InStock

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$210

Mouse monoclonal [LAT-01] antibody to LAT for WB, IP and Flow Cytometry (Intracellular).

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-LAT Antibody [LAT-01]

Description

Mouse monoclonal [LAT-01] antibody to LAT.

Specificity

This antibody reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.

Applications

WB, IP, Flow Cytometry (Intracellular)

Dilutions

IP: Tyrosine-phosphorylated form of LAT is not optimally recognized, Flow Cytometry: 1-4 µg/ml; Intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized, WB: 1-2 µg/ml.

Reactivity

Human

Cross Reactivity

This antibody does not cross react with Mouse.

Immunogen

Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.

Host

Mouse

Clonality

Monoclonal

Clone ID

LAT-01

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Predicted MW

36-38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

36 kDa phosphotyrosine adapter protein, Linker for activation of T-cells family member 1, p36-38, pp36

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Anti-LAT Antibody [LAT-01] (A86328)

Western blotting analysis of human LAT using Anti-LAT Antibody [LAT-01] (A86328)in lysates of Jurkat cells (positive) and Raji cells (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-LAT monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for LAT at approximately 38 kDa.

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Flow Cytometry - Anti-LAT Antibody [LAT-01] (A86328)

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using Anti-LAT Antibody [LAT-01] (A86328), (concentration in sample 1 µg/ml, GAM APC) and Anti-CD3 Antibody [UCHT1] (Pacific Blue), (20 µl reagent / 100 µl of peripheral whole blood).

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Flow Cytometry - Anti-LAT Antibody [LAT-01] (A86328)

Separation of human CD3 positive LAT positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained with Anti-LAT Antibody [LAT-01] (A86328), (concentration in sample 1 µg/ml, GAM APC).

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Flow Cytometry - Anti-LAT Antibody [LAT-01] (A86328)

Flow cytometry intracellular staining of human peripheral whole blood using Anti-LAT Antibody [LAT-01] (A86328), (concentration in sample 1 µg/ml, GAM APC).

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